Callus formation from full leaf and leaf parts of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne

Muhammet DOĞAN


Rotala rotundifolia (Buch-Ham. ex Roxb.) Koehne is a medicinal aquatic plant belonging to the family Lythraceae. Due to its ability to accumulate heavy metals, it also has a potential to be used for wastewater treatment. In this study, The effects  of the combinations of 0.05-0.25 mg/L Gibberellic acid (GA3) and 0.25 mg/L Thidiazuron (TDZ) on in vitro callus formation from the full leaf, upper half of leaf and lower half of leaf explants of R. rotundifolia were investigated. The highest percentage of callus formation (100%) from the full leaf, upper leaf and lower leaf explants were obtained on MS medium containing 0,25 mg/L GA3 and 0,25 mg/L TDZ, 0.05 and 0.25 mg/L GA3 + 0,25 mg/L TDZ and 0,05 mg/L GA3 + 0,25 mg/L TDZ. More callus concentrations were recorded in culture media containing GA3 at low concentrations. The shortest callus formation time was determined on the lower leaf explant cultured on MS medium containing 0.05 and 0.25 mg/L GA3 + 0.25 mg/L TDZ and on the 10th day. Various secondary metabolites from callus of R. rotundifolia using plant tissue culture techniques can be obtained and these metabolites can be used in the pharmaceutical field. In addition, callus formations can help gene transfer studies.


Gibberellic acid, In vitro, Callus, R.rotundifolia, Leaf explant

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Aasim M., Doğan M., Karataş M., Khawar K.M. 2017. In vitro direct whole plant regeneration of water lettuce (Pistia stratiotes L) using thidiazuron. Journal of Global Innovation in Agricultural and Social Sciences, 5: 1-4.

Ahmad N., Faisal M., Anis M., Aref I.M. 2010. In vitro callus induction and plant regeneration from leaf explants of Ruta graveolens L. South African Journal of Botany, 76: 597-600.

Anonim 2014. Rotala Rotundifolia. (erişim tarihi: 05.01.2018).

Arunyanart S., Chaitrayagun M. 2005. Induction of somatic embryogenesis in lotus (Nelumbo nucifera Geartn.). Scientia Horticulturae, 105: 411-420.

Bhowmik S., Saha M., Datta B.K. 2012. Extended distribution of Rotala rotundifolia (Buch.-Ham. ex Roxb.) Koehne (Lyhthraceae) from India. NeBIO, 3: 48-50.

Cheruvathur M.K., Sivu A.R., Pradeep N.S., Thomas T.D. 2012. Shoot organogenesis from leaf callus and ISSR assessment for their identification of clonal fidelity in Rhinacanthus nasutus (L.) Kurz., a potent anticancerous ethnomedicinal plant. Industrial Crops and Products, 40: 122-128.

Dogan M., Karatas M., Aasim M. 2015. An efficient in vitro plantlet regeneration of Ceratophyllum demersum L., an important medicinal aquatic plant. Fresenius Environmental Bulletin, 24: 3499-3504.

Dogan M., Karatas M., Aasim M. 2016. In vitro shoot regeneration from shoot tip and nodal segment explants of Pogostemon erectus (Dalzell) Kuntze, a multipurpose ornamental aquatic plant. Fresenius Environmental Bulletin, 25: 4777-4782.

Gentile L., Uccella N.A. 2014. Selected bioactives from callus cultures of olives (Olea europaea L. Var. Coratina) by LC-MS. Food Research International, 55: 128-136.

Giri L. Dhyani P., Rawat S., Bhatt I.D., Nandi S.K., Rawal R.S., Pande V. 2012. In vitro production of phenolic compounds and antioxidant activity in callus suspension cultures of Habenaria edgeworthii: A rare Himalayan medicinal orchid. Industrial Crops and Products 39: 1-6.

Ho Y.L, Huang S.S., Deng J.S., Lin Y.H., Chang Y.S., Huang, G.J. 2012. In vitro antioxidant properties and total phenolic contents of wetland medicinal plants in Taiwan. Botanical Studies, 53: 55-66.

Hoenemann C., Krueger K., Hohe A. 2012. Expression analysis of putative genes of pectin modifying enzymes in different callus lines of Cyclamen persicum and their correlation with the callus texture. Scientia Horticulturae, 140: 52-59.

Jayaraman S., Daud N.H., Halis R., Mohamed R. 2014. Effects of plant growth regulators, carbon sources and pH values on callus induction in Aquilaria malaccensis leaf explants and characteristics of the resultant calli. Journal of Forestry Research, 25: 535-540.

Karatas M., Dogan M., Emsen B. Aasim M. 2015. Determination of in vitro free radical scavenging activities of various extracts from in vitro propagated Ceratophyllum demersum L.. Fresenius Environmental Bulletin 24: 2946-2952.

Karataş M., Aasim M., Çınar A., Dogan M. 2013. Adventitious shoot regeneration from leaf explant of dwarf hygro (Hygrophila polysperma (Roxb.) T. Anderson). The Scientific World Journal, volume 2013, Article ID 680425, 7 pages.

Kumer Sen M., Nasrin S., Rahman S., Jamal A.H.M. 2014. In vitro callus induction and plantlet regeneration of Achyranthes aspera L., a high value medicinal plant. Asian Pacific Journal of Tropical Biomedicine, 4: 40-46.

Li H., Ye Z.H, Wei Z.J., Wong M.H. 2011. Root porosity and radial oxygen loss related to arsenic tolerance and uptake in wetland plants. Environmental Pollution, 159: 30-37.

Stanišić M., Raspor M., Ninković S., Milošević S., Ćalić D., Bohanec B., Trifunović M., Petrić M., Subotić A., Jevremović S. 2015. Clonal fidelity of Iris sibirica plants regenerated by somatic embryogenesis and organogenesis in leaf-base culture - RAPD and flow cytometer analyses. South African Journal of Botany, 96: 42–52.

Tan Q.G, Cai X.H., Feng T., Luo X.D. 2009. Megastigmane-type compounds from Rotala rotundifolia. Chinese Journal of Natural Medicines, 7(3): 187-189.

Tang W., Newton R.J., Charles T.M., 2006. Plant regeneration through multiple adventitious shoot differentiation from callus cultures of slash pine (Pinus elliottii). Journal of Plant Physiology, 163: 98-101.

Te-chato S., Lim M. 2000. Improvement of mangosteen micropropagation through meristematic nodular callus formation from in vitro-derived leaf. Scientia Horticulturae, 86: 291-298.

Turana S., Cornish K., Kumar S. 2014. Highly efficient callus-mediated genetic transformation of Parthenium argentatum Gray, an alternate source of latex and rubber. Industrial Crops and Products, 62: 212-218.


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