Determination of cytotoxic, genotoxic and oxidative effects of Naringenin

Selçuk ÇEKER, Furkan ORHAN, Güleray AĞAR


The phenolic compounds obtained from plants are responsible for the protection of the organism against the harmful effects of reactive oxygen compounds by means of antioxidant activities and for the treatment of disorders related to radical damage. However, since the phenolic compounds frequently found in plants may have antioxidant and prooxidant effects, the chemical properties and activities of these compounds must be known. Naringenin is of the most researched flavonoid members, it possesses majority of the biological activities of flavonoids. In this work, it is aimed to investigate the in vitro cytotoxic, genotoxic and oxidative effects of Naringenin, which is found in many foods in our diet.

The cytotoxic effect of Naringenin on human umbilical cord vein endothelial cells were analysed with the lactate dehydrogenase release and cell proliferation methods; in human lymphocytes the oxidative properties against carbon tetrachloride were examined with the measurement of catalase, superoxide dismutase and malondialdehyde; genotoxic effects were investigated with single cell gel electrophoresis and micronuclei methods.

Naringenin revealed cytotoxic and genotoxic properties especially at high concentrations (100 and 200 μM). The most effective results in terms of antigenotoxic and antioxidant effects for Naringenin was observed 8 μM concentration. Therefore, care should be taken that Naringenin does not consume food containing high levels unconsciously, and individual concentration adjustments should be made in the treatments applied.


Naringenin; Comet; LDH; Micronuclei; WST-1

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