DNA barcoding and phylogenetic analysis of Nelima pontica Charitonov, 1941 (Opiliones: Sclerosomatidae) based on mitochondrial COI and 16S rRNA genes



Nelima pontica is a species of Opiliones belonging to the family Sclerosomatidae. DNA barcoding was performed based on the mitochondrial, cytochrome oxidase I (COI) and 16S rRNA gene regions of this species and the gene sequences were identified. Basic Local Alignment Search Tool (BLAST) sequence analysis tool was used for the first time to search these sequences against the NCBI database. A phylogenetic tree was created by comparing these data with the data of Nelima which was previously existing record in the NCBI system.


Cytochrome Oxidase Subunit 1 (COI) Gene, Nelima pontica, phylogenetic analysis, 16S rRNA.

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Astrin JJ, Huber BA, Misof B, Klütsch CF. 2006. Molecular taxonomy in pholcid spiders (Pholcidae, Araneae): evaluation of species identification methods using CO1 and 16S rRNA. Zool Scr. 35:441–457.

Hebert PDN, Gregory TR. 2005. The Promise of DNA Barcoding for Taxonomy. Systematic Biology, 54, 852-859.

Kurt K. 2014. Updated checklist of harvestmen (Arachnida: Opiliones) in Turkey. Arch Biol Sci Belgrade 66: 1617-1631.

Kurt K. 2015. Harvestmen (Arachnida: Opiliones) Fauna of Surrounding Maçka (Trabzon) District. GÜFBED/GUSTIJ (2015) 5 (2): 114-123

Kury AB. 2013. Order Opiliones Sundevall, 1833. Zootaxa 3703: 27-33.

Snegovaya N, Marusik YM. 2012. New species and collection of Opiliones (Arachnida) Turkey. Acta Arachnologia, 61(2):59-70

Wang ZL, Yang XQ, Wang TZ, Xiaoping Yu. 2018. Assessing the effectiveness of mitochondrial COI and 16S rRNA genes for DNA barcoding of farmland spiders in China, Mitochondrial DNA Part A, 29:5, 695-702.

Wilson JJ. 2012. DNA barcodes for insects. Methods in molecular biology (Clifton, N.J.) 858:17-46


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