Haplotype variations of three Mitochondrial DNA genes in Microtus guentheri (Danford and Alston, 1880)

Derya ÇETİNTÜRK, Nuri YİĞİT, Ercüment ÇOLAK

Abstract

Synonym site mutations do not cause any changes in the protein sequence, whereas non-synonymous site mutations alter the protein sequence, causing amino acid changes. In this study, the rates of synonymous and non-synonymous substitution (Ks-Ka values) with the mutation and nucleotide variations in the synonymous and non-synonymous were analysed in Guenther’s vole Microtus guentheri (Danford and Alston, 1880) by amplification of three gene region (Cytochrome-oxidase I (COXI), Cytochrome-b (Cyt-b) and 12S rRNA), and haplotype variations were investigated in these genes. Haplotypes were found to be 12, 14 and 7 for the COXI, Cyt-b and 12S rRNA gene regions, respectively. Based on the Jukes and Cantor Parameter (Jukes and Cantor, 1969), the nucleotide diversity values in synonymous and non-synonymous regions were found to be close to each other in the COXI and Cyt-b regions but higher than in the 12S rRNA region. In addition to nucleotide diversity, Ka values in Cyt-b and 12S rRNA regions are generally higher than Ks values (Ka/Ks>1) while Ks and Ka values are very close to each other (Ks/Ka~1) in COXI region.

Keywords

Microtus guentheri, Mitochondrial DNA, Mutation.

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