Sublethal effects of chlorpyriphos on some hematological parameters in freshwater fish Anabas testudineus



Hematological parameters are often used to assess the health status and as stress indicators in fish. The aim of this study was to assess the sublethal effects of chlorpyriphos, a organophosphate insecticide, on some hematological parameters in freshwater fish Anabas testudineus. The effect was assessed based on the comparison results of control group and experimental groups exposed to sublethal concentrations (0.125, 0.250, 0.375  mg L-1) of chlorpyrifos at 7th, 14th and 21st days. It was observed that with the increase of chlorpyrifos concentrations and days, red blood cell counts, hemoglobin levels, hematocrit levels and thrombocyte counts decreased. White blood cell counts increased at 7th day of exposure but decreased with the increase of chlorpyrifos concentrations at 14th and 21th days. The study showed that the chlorpyrifos significantly impacted the health status of the fish and hematological parameters may be useful as a diagnostic test for chlorpyrifos exposure in A. testudineus.



chlorpyrifos, organophosphate insecticide, Anabas testudineus, hematological parameters

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Adedeji O.B., Adeyemo O.K., Agbede S.A. 2009. Effects of diazinon on blood parameters in the African catfish (Clarias gariepinus). African Journal of Biotechnology, 8(16): 3940-3946.

Banaee M., Mirvagefei, A.R., Rafei, G.R., Majazi, A.B. 2008. Effect of sublethal diazinon concentrations on blood plasma biochemistry. Intenational Journal of Environmental Research, 22: 189-198.

Frederick M.F. 2005. One of a series of the pesticides. Information Office, Florida Cooperative Extension service, Institute of Food and Agricultural Sciences, University of Florida.

Giron-Perez M.I., Barcelos-Garcia R., Vidal-Chavez Z.G., Romero-Banuelos C.A., Robledo-Marenco M.L. 2006. Effect of chlorpyrifos on the hematology and phagocytic activity of Nile tilapia cells (Oreochromis niloticus). Toxicology Mechanisms and Methods, 16: 495–499.

Haider M.J., Rauf A. 2014. Sub-lethal effects of diazinon on hematological indices and blood biochemical parameters in Indian carp, Cirrhinus mrigala (Hamilton). Brazilian Archives of Biology and Technology, 57(6): 947-953.

Jaffar Ali H.A., Rani V.J. 2009. Effect of phosalone on haematological indices in the Tilapia, Oreochromis mossambicus. Turkish Journal of Veterinary and Animal Sciences, 33(5): 407-411.

Joshi P.S., Deepa H., Manjushri B. 2002. Effect of lindane and malathion exposure to certain blood parameters in a freshwater teleost fish Clarias batrachus. Pollution Research, 21(1): 55-57.

Kaattari S.L., Piganelli J.D. 1996. The specific immune system: humoral defenses. In: G. Iwama, T. Nakanishi T (Ed.). The fish immune system. Organism, pathogen and environment. San Diego, CA, USA: Academic Press. pp. 207-254.

Kadam P., Patil R. 2016. Effect of chlorpyrifos on some biochemical constituents in liver and kidney of fresh water fish, Channa gachua (F.Hamilton). International Journal of Science and Research, 5(4): 1975-1979.

Koprucu S.S., Koprucu K., Ural M.S., Ispir U., Pala M. 2016. Acute toxicity of organophosphorous pesticide diazinon and its effects on behavior and some hematological parameters of fingerling European catfish (Silurus glanis). Pesticide Biochemistry and Physiology, 86: 99-105.

Levin E.D., Swain H.A., Donerly S., Linney E. 2004. Developmental chlorpyrifos effects on hatchling zebrafish (Danio rerio) swimming behaviour. Neurotoxicology and Teratology, 26: 719-723.

Luskova V. 1997. Annual cycles and normal values of haematological parameters in fishers. Acta Scientarum Naturalium Brno, 31(5): 70.

Maceda-Veiga A., Monroy M., Viscor G., De Sostoa A. 2010. Changes in non-specific biomarkersin the Mediterranean barbel (Barbus meridionalis) exposed to sewage effluents in a Mediterranean stream (Catalonia, NE Spain). Aquatic Toxicology, 100: 229–37.

Malla F.A., Sharma G., Singh S. 2009. Chlorpyrifos pesticide toxicity on erythrocyte sedimentation rate in fish, Channa punctatus (Bloch.). Biology and Medicine, 1(2): 54-55.

Mgbenka B.O., Oluah N.S., Umeike I. 2003. Effect of gammalin 20 (Lindane) on the differential white blood cell counts of the African catfish Clarias albopunctatus. Bulletin of Environmental Contamination and Toxicology, 71(2): 248–254.

Paracampo A., Solis M., Bonetto C., Mugni H. 2015. Acute toxicity of chlorpyrifos to the non-target organism Cnesterodon decemmaculatus. International Journal of Environmental Health Research, 25(1): 96-103.

Ramesh M., Saravanan M. 2008. Haematological and biochemical responses in a freshwater fish Cyprinus carpio exposed to chlorpyrifos. International Journal of Integrative Biology, 3(1): 80-83.

Reddy M.P., Bashamohideen M. 1989. Fenvalerate and cypermethrin induced changes in the haematological parameters of Cyprinus carpio. Acta Hydrochimica et Hydrobiologica, 17: 101-107.

Richards R.P., Baker D.B. 1993. Pesticide concentration patterns in agricultural drainage networks in the lake Erie basin. Environmental Toxicology Chemistry, 12: 13–26.

Sampath K., James R., Velammal S. 2003. Effect of methyl parathion on blood parameters and its recovery in a catfish, Mystus keletius. Indian Joyrnal of Fisheries Fish, 50(2): 191-197.

Satheeshkumar P., Ananthan G., Senthil Kumar D., Jagadeesan L. 2011. Haematology and biochemical parameters of different feeding behaviour of teleost fishes from Vellar estuary, India. Comparative Clinical Pathology, 1-5.

Sledge D., Yen J., Morton T., Dishaw L., Petro A., Donerly S., Linney E., Levin E.D. 2011. Critical duration of exposure for developmental chlorpyrifos-induced neurobehavioral toxicity. Neurotoxicology and Teratology, 33: 742–751.

Svobodam M., Luskova V., Drastichova J., Zlabek V. 2001. The effect of diazinon on haematological indices of common carp (Cyprinus carpio L). Acta Veterinaria Brno, 70: 457-465.

Thomas K., Nicholson B.C. 1989. Pesticide losses in runoff from a horticultural catchment in South Australia and their relevance to stream and reservoir water quality. Environmental Technology Letters, 10: 117–129.

Velmurugan B., Selvanayagam M., Cengiz E.I., Bilici S., Satar A. 2015. Scanning electron microscopy study of the gills, scales and erythrocytes of Anabas testudineus upon exposure to chlorpyrifos. Toxicological and Environmental Chemistry, 97(2): 208-220.

WHO (World Health Organization). 2003. Chlorpyrifos in drinking-water. Background document for preparation of WHO Guidelines for drinking-water quality. Geneva.

Yagi A.J., Auta J. 2007. Sublethal effects of monocrotophos on some haematological indices of African Catfish Clarias gariepinus. Journal of Fisheries International, 2: 115-117.


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